In vitro fertilisation in 10 steps!
1. Ovarian stimulation to induce the growth of multiple ovarian follicles using appropriate drugs, with medication personalised by the responsible doctor for the specific woman.
2. Measuring ovarian follicle growth using ultrasound.
3. Measuring oestradiol, LH, and progesterone in the bloodstream, in order to assess ovarian follicle growth and maturation.
4. Induction of final maturation of the ova by administering an hCF or GnRH- agonist and ova harvesting 36 hours later.
5. Ova collection intracervically using a paracentesis needle under ultrasound monitoring. The procedure is performed under sedation to avoid any pain.
6. Sperm is collected in a sterilised container through masturbation and must be quickly delivered to the laboratory.
7. The sperm undergoes special laboratory processing so as to decide which method will be followed. The ICSI method is applied in cases of men with sperm problems (oligospermia, asthenospermia, teratospermia, azoospermia).
8. In IVF the ovum/ova are fertilised by the spermatozoa at the laboratory, in special dishes containing nutrients, which are incubated, and their fertilisation and cell-division is monitored.
In the ICSI method one spermatozoon is placed inside an ovum and they are incubated while their fertilisation and division is monitored.
9. Embryo transfer is performed under ultrasound monitoring, after the the embryo or embryos have been placed inside the uterine cavity. The process is brief and performed without anaesthesia.
10. Subsequently, the luteal phase is enhanced with the use of natural progesterone in the form of vaginal repositories/tablets or subcutaneous hCG injections. A pregnancytestisperformed 12-14 dayslater.